Atg9 localized onto the autophagosomal membranes. Video associated with Fig. 4 D. ATG9-2×GFP ypt7Δ cells and ATG9-2×GFP ypt7Δ atg11Δ atg17Δ cells were starved for 4 h. Images were analyzed by time-lapse microscopy at 32 ms/frame using an inverted microscope (IX71). Nut., nutrient; Stv., starved.