Video 8.

Atg9 vesicles assembled individually to the PAS. Video associated with Fig. 4 C. ATG9-2×GFP atg1^D211A-mCherry atg11Δ cells were treated with rapamycin for 1 h. Images were analyzed by time-lapse microscopy at 32 ms/frame using an inverted microscope (IX71).

Atg9 vesicles are an important membrane source during early steps of autophagosome formation

Hayashi Yamamoto, Soichiro Kakuta, Tomonobu M. Watanabe, Akira Kitamura, Takayuki Sekito, Chika Kondo-Kakuta, Rie Ichikawa, Masataka Kinjo, and Yoshinori Ohsumi

DOI: 10.1083/jcb.201202061