Mislocalization of Atg9 in atg23Δ or atg27Δ cells. Video associated with Fig. 3 C. ATG9-2×GFP atg11Δ atg17Δ cells lacking Atg23 and/or Atg27 were treated with rapamycin for 3 h. Images were analyzed by time-lapse microscopy at 32 ms/frame using an inverted microscope (IX71). WT, wild type.