Video 2.

The motion of Atg9 vesicles was not altered after the treatment with latrunculin A. ATG9-2×GFP ABP140-mCherry cells were treated with 100 µg/ml latrunculin A (LatA) for 20 min. Images were analyzed by time-lapse microscopy at 32 ms/frame using an inverted microscope (IX71).


Atg9 vesicles are an important membrane source during early steps of autophagosome formation

Hayashi Yamamoto, Soichiro Kakuta, Tomonobu M. Watanabe, Akira Kitamura, Takayuki Sekito, Chika Kondo-Kakuta, Rie Ichikawa, Masataka Kinjo, and Yoshinori Ohsumi

DOI: 10.1083/jcb.201202061
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