The intensity of Atg9-2×GFP clusters on the autophagosomal membrane was apparently comparable to that of the cytoplasmic mobile Atg9-2×GFP vesicles. ATG9-2×GFP ypt7Δ atg11Δ cells used in Fig. 6 D were starved for 3 h in SD(–N) medium. Images were analyzed by time-lapse microscopy at 30 ms/frame using an inverted microscope (IX71). The intensities of clusters on the autophagosomal membrane were apparently comparable to those of the cytoplasmic mobile Atg9 vesicles.
