Localized dendritic translocation of αCaMKII correlates with spine growth. Time-lapse imaging of mGFP-αCaMKII (top) and mCherry (down) in a spine and subjacent dendrite stimulated with 0Mg2+/Gly for 5 min. During the stimulation, mGFP-αCaMKII first accumulates into the spine and then in the dendritic segment at its base. The spine gradually grows and its increase in size persists 30 min after the stimulation. Frames were taken every 10 s for the first 8 min and then every 30 s with a CoolSnap-HQ camera (Photometrics) on an Axioskop FS2 microscope (Achroplan 63x 0.95 NA objective; Carl Zeiss). Related to Fig. 7.
