Cell-wide depolarization induces αCaMKII translocation to microtubular elements throughout the neuron. Time-lapse imaging of a neuron expressing mGFP-αCaMKII stimulated with 40 mM KCl for 1 min. During the stimulation, mGFP-αCaMKII translocates to synapses throughout the neuron, but also to microtubules in the somatodendritic area. Frames were taken every 10 s for the first 4 min and then every 30 s with a CoolSnap-HQ camera (Photometrics) on an Axioskop FS2 microscope (Achroplan 63x 0.95 NA objective; Carl Zeiss). Related to Fig. 3.
