αCaMKII translocates to dendritic sites of higher Ca2+ concentration. Time-lapse imaging of a neuron expressing both mCherry-αCaMKII (left) and GCaMP2 (right), a genetically encoded Ca2+ indicator, and stimulated with 0Mg2+/Gly for 5 min. Ca2+ oscillations during the stimulation lead to the gradual accumulation of mCherry-αCaMKII at sites where Ca2+ amplitude and/or frequency are higher (examples are pointed by the arrows). Frames were taken every 2 s with a QuantEM:512SC camera (Photometrics) on an Axioskop FS2 microscope (Achroplan 63x 0.95 NA objective; Carl Zeiss). Related to Fig. 2.
