Video 5.

Non-cross-linked GCN4-Kar3Vik1 promoted microtubule minus-end-directed gliding representative. TIRF microscopy time-lapse movie of polarity-marked rhodamine-microtubule gliding during a 45-min period with frames collected every 20 s (AxioObserver Z1 Inverted Laser TIRF3 microscope system; Carl Zeiss). The running time is in minutes:seconds with video playback at 280× real-time speed. Related to Fig. 7.

Kar3Vik1, a member of the Kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern

Katherine C. Rank, Chun Ju Chen, Julia Cope, Ken Porche, Andreas Hoenger, Susan P. Gilbert, and Ivan Rayment

DOI: 10.1083/jcb.201201132