Cross-linked SHD-Kar3Vik1 promoted microtubule minus-end-directed gliding representative. TIRF microscopy time-lapse movie of polarity marked rhodamine-microtubule gliding during a 45-min period, with frames collected every 20 s (AxioObserver Z1 Inverted Laser TIRF3 microscope system; Carl Zeiss). The running time is in minutes:seconds with video playback at 280× real-time speed. Related to Fig. S1 and Fig. 3.
