EM tomogram of a kar1-Δ15 cell. Digital images were taken at 300 kV from a −60° to 60° tilt with 1° increment on an electron microscope (Tecnai F30) equipped with an Eagle 4k charge-coupled device camera (pixel size of 1.499 nm at a 15,500 magnification). Tomograms were then generated by R-weighted back projection, modeled, and analyzed using the IMOD software package.
