3D reconstruction of a kar1-15 cell. 3D model of an α-factor–treated kar1-Δ15 cell containing three single detached cMTs (see Fig. S4). Green indicates MTs, purple indicates the nuclear envelope, blue indicates the plasma membrane, yellow indicates the SPB, and dark yellow indicates mitochondria. MT minus ends are labeled with red dots. Using 3dmod (IMOD package), we generated a TIFF image sequence of a full 360 Y-rotation of the reconstructed model (500 frames). These TIFF images were imported into ImageJ, and a video was generated with a speed of 7 frames/s and compressed in jpg.
