Localization of PIs during cell–cell fusion with long projections. RAW264.7 cells expressing the reporter constructs in Fig. 1 A in the presence of 1 µg/ml Dox were stimulated with 10 ng/ml RANKL for 48 h. Phase-contrast images were also obtained. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (FluoView FV10i). Frames were taken every 2 min for 34 min. Bar, 25 µm.
