Localization of PIs during filopodium-mediated cell–cell fusion. RAW264.7 cells expressing the reporter constructs in Fig. 1 A in the presence of 1 µg/ml Dox were stimulated with 10 ng/ml RANKL for 48 h. Cells were exposed to DAPI at 1 µg/ml (blue) for 1 h before observation to visualize nuclei. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (TCS SP5). Frames were taken every 2 min for 10 min. Bar, 25 µm.
