CK666 (50 µM, 20 min) increases the peripheral retrograde F-actin flow rate. FSM time lapse of F-actin dynamics before and after 20 min in CK666 recorded from an Aplysia bag cell neuron growth cone in a cell injected with trace levels of Alexa 568 G-actin. Images were acquired with a spinning disk system (Revolution XD; Andor) using a confocal head (CSU-X1; Yokogawa) coupled to an inverted microscope (TE 2000E; Nikon) equipped with Perfect Focus. Images were recorded with an EMCCD camera (iXonEM+ 888; Andor). Bar, 5 µm. 5-s interval; elapsed time, 2 min; playback, 8 frames per second. This movie is from the growth cone in Fig. 4 A.
