Simultaneous topographical HPICM and fluorescence confocal imaging of CCPs in the membrane of a live Cos-7 cell. Topographical HPICM imaging (left) of a Cos-7 cell transfected with Сlc-EGFP (right; green). The scan size is 2 × 2 µm. Frames were acquired at 15 s/frame. The experiment was performed at 28°C.