Actin cytoskeletal dynamics at stable and remodeling junctions. Time-lapse recording of HUVECs expressing VE-cadherin–GFP (green) and Lifeact-mCherry (red). The left side shows a stable junction; VE-cadherin remains aligned by thick parallel actin bundles that do not overlap with the junction. The right side shows the dynamics of the actin cytoskeleton at a perpendicularly oriented remodeling junction. Perpendicularly oriented VE-cadherin areas are attached to radial actin bundles from both cells participating in the cell–cell junction. Images were acquired by time-lapse wide-field microscopy (Eclipse Ti; Nikon) using a 60× 1.49 NA oil objective lens. Frames were taken every 30 s for 52 min.
