Actin dynamics and loss of anchors in response to cessation of flow. A representative Lifeact-GFP–transfected U937 cell was adhered to a VCAM-1–coated surface and exposed to fluid flow (2 dynes/cm2; right to left) for 2 min. The video begins immediately after fluid flow stopped (t = 0 s). Within 40 s, the upstream anchors (on the right side of the cell) diminish in size, and F-actin (green) that was accumulated in the upstream cortical actin cytoskeleton gradually decreases (FluoView 1000; zoom factor: 7.0×; frame rate: 0.829 s/frame). (left) GFP image; (right) DIC image.
