Eg5 activity is required to maintain extra foci of microtubules in cells expressing mTPX2-710LAP. An LLC-Pk1 cell expressing mTPX2-710LAP and mCherry–α-tubulin and treated with pTPX2 siRNA and 200 µM monastrol. Images were acquired using a spinning disk confocal microscope. Frames were acquired at 1.5-min intervals for 23 min. The video corresponds to Fig. 5.
