Formation of extra foci of microtubules in cells expressing mTPX2-710LAP. An LLC-Pk1 cell expressing mTPX2-710LAP and mCherry–α-tubulin and treated with pTPX2 siRNA to deplete endogenous TPX2. Images were acquired using a spinning disk confocal microscope. Frames were acquired at 1-min intervals for 24 min. The video corresponds to Fig. 2 B (top).
