Conjugate formation between an SEE-pulsed Raji cell (CMAC; blue) and a control (EV) Jurkat cell transfected with centrin-GFP (green). The fluorescence is superimposed on grayscale brightfield images to illustrate conjugate formation. Images were analyzed by time-lapse confocal microscopy using a spinning-disk confocal microscope (PerkinElmer). Note that time 0 in Fig. 2 A refers to first frame after conjugate formation and not to the start of the video. Frames were taken every 3 min for 102 min. The display rate is two frames per second.
