3D time-lapse imaging reveals ZYG-12::GFP patch motions along the NE. The first sequence (2–4 s) shows 3D time-lapse imaging of a hermaphrodite worm expressing ZYG-12::GFP. The field of view spans the entire TZ region of the gonad shown in Fig. 1 C, with premeiotic nuclei shown on the left and some nuclei that have progressed into pachytene shown on the right. Projections of stacks of optical sections acquired every 2 s on the OMX imaging system are played back at 25 fps (50× real time). Bar, 5 µm. The second sequence (from 6 s to the end) shows a rotating animation of the same dataset to highlight the 3D image reconstruction. Large tick marks indicate 5 µm. Time is given in minutes and seconds.
