Occurrence of C-type apoptotic cells after the midline fusion. Time-lapse video of apoptosis during and after the midline fusion in MHNP closure, as shown in Fig. 3 F. C-type apoptosis abundantly occurred after sealing of the midline by closure II. Some of the cells undergoing C-type apoptosis in the midline are shown by white arrowheads. Caspase activation is indicated by the V/C (1.0–0.5) using pseudocolor. Live imaging was performed on an inverted confocal microscope (TCS SP5). Frames were taken at intervals of 4 min for 12 h.
