Accumulation of CA-KIF5C to the tips of a future axon in MAP2 ( −/ −) hippocampal neurons in stage 2-to-3 transition. Hippocampal cells from MAP2 ( −/ −) embryos were cultured and transfected with K381-YFP cDNA by electroporation. Time-lapse images were obtained at 37°C at 10-min intervals, from 36 h after dissociation using an autofocus multiple-point inverted microscope (IX-72 ZDC; Olympus) equipped with a cooled CCD camera. Note, K381-YFP switches neurites at stage 2 and accumulates to axonal tips at stage 3 (arrows), similar to wild-type neurons.
