Time-lapse movie of fluorescence micrographs of an adf1-1 cell expressing Rlc1p-3GFP at 36°C. Images are maximum intensity projections (ImageJ) of Z-series of confocal stacks at each time point, acquired with a microscope (model IX-71; Olympus) equipped with a spinning-disk confocal unit (Ultra-view RS; PerkinElmer) and an Orca-ER camera (Hamamatsu Photonics). Nodes coalesced into a big clump (Fig. S1 C shows the same cell). Time in minutes.
