MT plus end growth visualized with EB3-GFP in control, SLAIN2-, and ch-TOG–depleted cells. HeLa cells stably expressing EB3-GFP were imaged using epifluorescent microscopy on a research microscope (Eclipse Ti-E) with a 0.5-s exposure time without delay between frames for 50 s. Left, control; middle, SLAIN2 siRNA#2; right, ch-TOG siRNA.