Video 4.

MT plus end growth visualized with EB3-GFP in control, SLAIN2-, and ch-TOG–depleted cells. HeLa cells stably expressing EB3-GFP were imaged using epifluorescent microscopy on a research microscope (Eclipse Ti-E) with a 0.5-s exposure time without delay between frames for 50 s. Left, control; middle, SLAIN2 siRNA#2; right, ch-TOG siRNA.


SLAIN2 links microtubule plus end–tracking proteins and controls microtubule growth in interphase

Babet van der Vaart, Cristina Manatschal, Ilya Grigoriev, Vincent Olieric, Susana Montenegro Gouveia, Saša Bjelić, Jeroen Demmers, Ivan Vorobjev, Casper C. Hoogenraad, Michel O. Steinmetz, and Anna Akhmanova

DOI: 10.1083/jcb.201012179
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