The effect of CCE pretreatment on exocytosis from a PMA-stimulated endothelial cell. HUVECs coexpressing GFP-VWF (green) and the mCherry–P-selectinLum domain (red) were pretreated with 1 µM CCE for 15 min before stimulation with 100 ng/ml PMA and visualization by spinning-disk confocal microscopy (UltraVIEW VoX). Z stacks of 14 0.5-µm slices were acquired every 5 s for 10 min, and the image shown is a maximum intensity projection. The video is shown at seven frames per second. Note the increased incidence of fusion, but GFP-VWF fails to fully exit the cell.
