PMA-stimulated endothelial cell. HUVECs coexpressing GFP-VWF (green) and the mCherry–P-selectinLum domain (red) were stimulated with 100 ng/ml PMA and visualized by spinning-disk confocal microscopy (UltraVIEW VoX). Z stacks of 13 0.5-µm slices were acquired every 5 s for 10 min, and the image shown is a maximum intensity projection. The video is shown at seven frames per second. Note fusion events typified by loss of mCherry signal and subsequent exocytosis of GFP-VWF.
