Different plasma membrane components are involved in the cell wound repair process. (A) GFP-Spider and (B) GAP43 are dynamically recruited to cell wounds. (C) Fluorescence intensity analysis of GAP43-ChFP recruitment to the cell wound. Single fluorescence images in were captured with a spinning disk confocal system (PerkinElmer) on an inverted microscope (TE2000-E; Nikon). Frames were captured at 30-s intervals for 10 min followed by 1-min intervals for 30 min. Bar, 20 µm.
