Actin and microtubule cytoskeleton are required for normal cell wound repair. (A) Actin is required for cell wound repair. Time-lapse confocal microscopy of a wounded early embryo treated with Lat B and expressing actin (SGMCA). Images were acquired using a spinning disk confocal system (PerkinElmer) with an inverted microscope (TE2000-E; Nikon), captured at 45-s intervals for 3 min followed by 1-min intervals for 18 min. (B) The microtubule cytoskeleton is required to establish the actin ring at the wound edge. Images of a wounded early embryo expressing actin (sGMCA) and treated with colchicine were analyzed using a spinning disk confocal system (PerkinElmer) with an inverted microscope (TE2000-E; Nikon). Frames were captured at 30-s intervals for 10 min followed by 1-min intervals for 18 min. Bar, 20 µm.
