3D reconstruction of the staining pattern of Hts-RC in an rngo mutant egg chamber at stage 8; related to Fig. 4. 3D reconstruction of a Drosophila egg chamber at stage 8 containing an rngoGE3956 germline clone, which was stained for Hts-RC (white). Note that only rudimentary ring canals are visible and that Hts-RC is spread over the whole plasma membrane of germline cells. The reconstruction is derived from still images obtained using a confocal microscope (LSM 510 Meta).