Centrosome movements in a NudE/EL-depleted prophase cell. A HeLa cell stably expressing EB3-GFP (microtubules plus tips, green) and H2B-mCherry (chromosomes, red) and transfected with NudE and NudEL siRNA duplexes imaged from prophase to metaphase. Time is in minutes. Note that unsplit centrosomes were already detached from the NE at the beginning of this video. Video is related to Fig. 5 Ad.
