Video 2.

Live imaging of VMAT2-pHluorin basal and regulated exocytosis in PC12 treated with AP-3 δ siRNA. PC12 cells were transfected with 50 nM AP-3 δ siRNA, cotransfected 2 d later with the same siRNA and VMAT2-pHluorin, then imaged live by TIRF microscopy after an additional 2 d as described for Video 1.

RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway

Cédric S. Asensio, Daniel W. Sirkis, and Robert H. Edwards

DOI: 10.1083/jcb.201006131