HUVEC migration on 8.7 kPa 2D PA substrates. Montage of time-lapse movies of HUVECs treated with either DMSO control (left), 20 µM Taxol (middle), or 20 µM nocodazole (right) were acquired a spinning disc confocal microscope (TE2000; Nikon) and a 20× 0.45 NA objective lens (Nikon). Images were acquired for 15 h (with a frame taken every 15 min) beginning 60 min after addition of the drug. Bar, 50 µm.
