3D reconstruction of invasive FCM-specific F-actin foci and the corresponding cup-shaped dimple on founder cell membrane. A 3D reconstruction of three F-actin foci (green) in FCMs (blue) invading a founder cell to create cup-shaped dimples (red). Stage 15 wild-type embryo triple labeled with phalloidin (green), α-Lmd (blue), and α-Duf (red). The walls of the cups are composed of enriched Duf protein, and the cup is filled with dense F-actin. The blue staining of FCMs is only shown in the first and last frames.
