Subcellular dynamics during interkinetic nuclear movements and mitotic cleavages. Time-lapse video of interkinetic nuclear movements and mitotic cleavages of THN progenitors starting at ∼30 hpf. Lateral view of the cerebellar anlage of a Tg(atoh1a:Gal4TA4)hzm2 transgenic zebrafish embryo injected with vector M1 at the one-cell stage. Images are maximum projections of z-stacks (spacing = 1.8 µm; 26 planes) recorded every 404.2 s using a 40x water objective and a confocal microscope (LSM 510; Carl Zeiss, Inc.). Images were rendered using Adobe Photoshop CS2 and ImageJ. The video (2 frames/sec) was animated using QuickTime Pro. This video shows that the centrosome strictly localizes to the apical site in THN progenitors undergoing INM. Centrosome duplication in mitotic cells can be observed as well as centrosome relocation to the apical site after mitosis.
