Wound healing: loss of cell–cell contacts and enhanced invasion capacity. 11-h time course of unsorted CHO–EGFP-BRCA11634–1863 (CHO-EGFP) cells, imaged at 2-min intervals and sampled every two frames, showed the enhanced invasion capacity of single cells (orange track). The pink tracks point at loss of cell–cell contact and detachment of cells that are migrating away from the front line into the wound. Tracks were obtained using ImageJ and MTrackJ (National Institutes of Health). Playback speed, 12 frames per second. Nonconfocal phase contrast and EGFP fluorescence merged images are shown.
