Video 10.

PI-103 selectively inhibits PI(3,4,5)P₃ formation without affecting PI(3)P formation. Time-lapse video showing a macrophage transiently expressing PH-Akt–GFP and 2FYVE-RFP. Addition of 100 nM PI-103 abolished the recruitment of PH-Akt without affecting the localization of 2FYVE-RFP. 40 s per frame; 35-min duration.

Class I and class III phosphoinositide 3-kinases are required for actin polymerization that propels phagosomes

Michal Bohdanowicz, Gabriela Cosío, Jonathan M. Backer, and Sergio Grinstein

DOI: 10.1083/jcb.201004005