Normal human fibroblasts contain the influx of FM1-43 after SLO permeabilization. Normal control fibroblasts were preincubated or not with 600 ng/ml SLO at 4°C and transferred to a live imaging chamber at 37°C, followed by the addition of FM1-43 and time-lapse confocal imaging for 4 min at 1 frame/3 s. (A) No SLO was added to cells in Ca2+-containing DME. (B) Ca2+-free DME and FM1-43 were added to SLO-treated cells 10 s before the beginning of the video. (C) Ca2+-containing DME and FM1-43 were added to SLO-treated cells 10 s before the beginning of the video. This video is displayed at 8 frames/s. Bar, 18 µm.
