Viral collision with the nuclear periphery. EGFP-actin is shown in High Five cells 5 min after infection with an MOI of ∼200 and 2 d after transfection with the EGFP-actin–expressing plasmid. The yellow circle highlights a virus colliding with the nucleus, although other collisions are also apparent. After collisions, virus-associated actin comet tails persist, resembling corkscrews that radiate from the nuclear periphery. Images were captured every 5 s for 13.3 min by time-lapse epifluorescence microscopy and are displayed at 10 frames/s. Bar, 5 µm.
