Spindle severing in a let-99 embryo. Vertical white bar at t = 65 s marks the time and position of laser ablation of the central spindle. Images in the time-lapse sequence were acquired at a 1-s interval; the time stamp shows elapsed time, where t = 0 is the first frame of the video. Bar, 10 µm. The video playback rate is 12 frames per second.