CHC and/or TACC3 siRNA-treated HeLa cells failed to proceed into anaphase over 5 h from chromosome condensation onset. HeLa cells were transfected with the indicated siRNAs along with GFP-H2B for chromosome observation. Images were analyzed by time-lapse microscopy using an inverted microscope. Frames were taken every 5 min for 6 h. The video is shown at 5 frames/s. Bars, 10 µm.
