Depletion of KHC abolishes peroxisome transport toward both the plus and minus ends of microtubules. Sequence of a CytoD-treated Drosophila S2 cell from Fig. 1 B (middle). The cell is expressing peroxisome-targeted GFP (GFP-SKL) in a KHC-depleted background. Images were captured at 1 frame/s over 1 min (see Materials and methods). Playback speed is 8 frames/s.