Centrosome ablation in sand dollar blastomeres coexpressing GFP-rGBD (red) and ensconsin-3xGFP (cyan). Time-lapse sequences of single confocal sections (Atto CARV). First segment ( Fig. 8 B): two single ablations surrounded by normal cells. Second segment ( Fig. 8 D): one normal, one singly ablated, and one doubly ablated cell. Third segment ( Fig. 8 C): doubly ablated cell. Real times are indicated, and the video is encoded at 15 frames/s. Bars, 25 µm.
