Daughters of a single SSC dynamically sample the same microenvironment following their birth and before they divide again to form two clones of germ cells with distinct fates. Cultured GCS-EGFP rat germ cells (green) were freshly plated right before imaging, and maintained at 37°C with humidity control and 5% CO2 perfusion during time-lapse imaging by a DeltaVision RT microscope (Applied Precision) for 18 d. Frames shown were taken every 30 min. Approximately 98 h are shown in this video. The mother SSC is labeled by a blue dot. After the mother SSC’s division at the eighth hour, one of its daughter cells is labeled with a blue dot, and the other with a green dot. A fate map of these two daughter cells’ progeny is shown in Fig. 4 B, and the quantitative analysis of their relative positions over time is shown in Fig. 4 (C and D). The time compression ratio is 43,160:1.
