Distribution of PS during phagosome formation and maturation. RAW macrophages were transfected with the PS biosensor GFP-Lact-C2 and exposed to IgG-opsonized sRBCs. Confocal images were acquired for 24 min 47 s, as indicated by the timer display. Note that the phagosome formed near the top of the cell, which undergoes extensive interactions with GFP-Lact-C2–positive vesicles and tubules. The video is shown at 12 frames per second.
