3D reconstruction of three jejunal villi from a transgenic mouse. This video was created from a single z series. Both villous structure and localization of fluorescent tight junction proteins EGFP-occludin and mRFP1–ZO-1 in the epithelium can be appreciated in these images. EGFP-occludin is shown in green, mRFP1–ZO-1 is shown in red, and nuclei are shown in blue. The luminal space appears black. The images were collected every 0.1 µm. A single image from this video is shown in Fig. 2 D.
