Spindle rotation in HeLa Kyoto cells depleted of hSpindly (hSpindly-2 siRNA) and dynein heavy chain (DHC). Time-lapse imaging of HeLa Kyoto cells stably expressing GFP-α-tubulin/cherry-H2B treated with hSpindly-2+DHC siRNA and exposed to MG132. Imaging was performed using a 40x objective and images were captured using 10- and 80-ms exposures for cherry and EGFP, respectively, every 3 min for 16 h.