Localization of GFP-Polo in wild-type embryos. A wild-type embryo as it progresses through cycle 12 is shown. Notice the movements of GFP-Polo into the nucleus and to the kinetochores in conjunction with mitotic onset and centrosome splitting as the poles reach maximum separation in late anaphase. This video corresponds to Fig. 1 A (video is a projection of five confocal sections 1.5 µm apart; frames are 48 s apart initially and subsequently 26 s to minimize photobleaching). Bar, 7 µm.
