Slimb colocalizes with the centriole marker SAS-6 in S2 cells. An S2 cell stably expressing both Slimb-EGFP (green) and mCherry–SAS-6 (red), which labels centrioles (arrow). The video is presented in four panels (from left to right): Slimb-EGFP (monochrome), mCherry–SAS-6 (monochrome), merged view with Slimb in green and SAS-6 in red, and a higher magnification of the boxed region shown in the merged view. Centrioles in these cells are not stationary but instead constantly spin and move apparently randomly throughout the cell (Rogers, G.C., N.M. Rusan, M. Peifer, and S.L. Rogers. 2008. Mol. Biol. Cell. 19:3163–3178). Thus, any perceived asymmetry in Slimb localization on centrioles is the result of a delay in image acquisition using live cell microscopy. Frames were acquired every 2 s for 2 min and are displayed at 8 frames/s.
